SkiBum

PSP 10162

DNA Source: Beach Party

General Information

Accession Date
December 3, 2025
Report Type
Whole-Genome Sequencing
Clonal or not?

Not yet assessed: this sample was not in the latest collection comparison.

Clone Test

Is SkiBum a resequencing of a culture already in Psilocydia? Every assembled genome was aligned directly to its closest genome in the collection and the divergence measured. Pairs that are one culture sequenced twice sit at or below about 0.03%; genuinely different cultures sit well above it. Where SkiBum falls on that scale answers the question, calibrated against the collection's own resequencings rather than a rule of thumb.

SkiBum was not in the latest collection comparison (no assembly yet). The comparison is refreshed with each pipeline run.

Genetic Information

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Blob Plot

Variants

Changes in the psilocybin genes were found by two independent analysis methods applied to this sample's sequencing reads: aligning the reads to the reference genome, and assembling the genome from them. Each change is labelled with the method that found it, or Both; hover a label for the read counts.

How we found these
Both
In the assembly and in the reads, so confirmed twice.
Reads only
In the reads, not in the assembly. The hover says why where we know: the assembly has the reference base there, does not cover the site, or this sample has no assembly. Many of these are changes carried by only part of the reads; an assembly keeps one base at each position.
Assembly only
In the assembly, but the reads show the reference base there, or too few reads cover the site to say.

Read counts. At every site we count the reads carrying each base (reads with mapping quality of at least 20, bases with quality of at least 13; 8 reads or more to say anything). The change is in most reads at 80% or more, mixed between 20% and 80%, in a minority from 5% to 20% (and at least 2 reads), and essentially absent below that. These counts say what the reads show; they say nothing about how many copies of the gene the sample carries. A change counts as found in the reads when these counts show it (in most, mixed or a minority of reads) or when the lab's variant report lists it. That report lists only changes its gene models rate HIGH or MODERATE, so many changes the reads show are not on it.

Names. Protein changes are named on our curated gene models for the psilocybin cluster. The read-alignment report names them on the public reference's gene models, which in places differ (for example, the public models join psiT1 and psiK into one gene); where the names differ, the hover shows both.

What is listed. Changes that alter the protein (rated HIGH or MODERATE), and every change on the lab's variant report for the cluster. Positions are on chromosome 10 of the reference genome.

psiT1 1 variant — 1 MODERATE
psiT1
MODERATE E121G p.Glu121Gly missense A/G Both
chr10:1663786 73 of 149 reads
psiK 1 variant — 1 MODERATE
psiK
MODERATE R173G p.Arg173Gly missense A/G Both
chr10:1666622 106 of 203 reads
psiH 1 variant — 1 MODERATE
psiH
MODERATE S7F p.Ser7Phe missense C/T Both
chr10:1667530 135 of 271 reads
psiM 1 variant — 1 MODERATE
psiM
MODERATE R102K p.Arg102Lys missense C/T Both
chr10:1674573 106 of 183 reads
psiD 2 variants — 2 MODERATE
psiD
MODERATE G206N p.Gly206Asn missense CC/TT Both
chr10:1676885 73 of 133 reads
psiD
MODERATE L22F p.Leu22Phe missense C/A Reads only
chr10:1677485 93 of 177 reads

Genetic Relatives

Nearest

  1. Mexican_Dutch_King-1-PS (PSP10070) — 0.211
  2. Thai-1-PS (PSP10053) — 0.245
  3. Golden-Teacher-ITW-WM (PSP10130) — 0.294
  4. Orissa-India-4-Mush (PSP10073) — 0.325
  5. Blue-Magnolia-Rust (PSP10086) — 0.326
  6. Orissa-India-1-Mush (PSP10072) — 0.333
  7. BM2-UM-WM (PSP10126) — 0.336
  8. DNA-PA1-15SEPT2022 (RSP12858) — 0.336
  9. DNA-PA1-6mCryo-15SEPT2022 (RSP12857) — 0.341
  10. Golden-Teacher_Denver (PSP10011) — 0.343
  11. BM1-UM-WM (PSP10124) — 0.345
  12. Thailand-Koh-Samui-1-Mush (PSP10080) — 0.348
  13. Penvy-2-SW (PSP10075) — 0.349
  14. Penvy-Col4-PS (PSP10043) — 0.349
  15. Penvy-1-PS (PSP10045) — 0.350
  16. Penvy-Col5-PS (PSP10044) — 0.350
  17. MGC_2016_unknown_strain (PSP10012) — 0.360
  18. BM16 (PSP10087) — 0.368
  19. KOH-Samui-1-ITW (PSP10063) — 0.370
  20. Blue-Magnolia-Rust-UM-WM (PSP10127) — 0.370

Most Distant

  1. DNA-PG-15SEPT2022 (RSP12862) — 0.515
  2. DNA-PF-15SEPT2022 (RSP12861) — 0.511
  3. DNA-PB-15SEPT2022 (RSP12859) — 0.507
  4. Albino-Aplus-1-SW (PSP10013) — 0.498
  5. Amazon-ITW-WM (PSP10122) — 0.496
  6. Blue-Meanie-ITW-WM (PSP10128) — 0.496
  7. Aplusplus (PSP10085) — 0.474
  8. BplusONT-WM (PSP10141) — 0.471
  9. Bplus_Denver (PSP10010) — 0.469
  10. Albino-Aplus-3-PS (PSP10000) — 0.464
  11. AAplus-4-Mush (PSP10057) — 0.463
  12. Panaeolus-Copelandia-Tropicalis-2-Mush (PSP10077) — 0.460
  13. BM1b-UM-WM (PSP10125) — 0.458
  14. Jedi_Mind_Fuck (PSP10158) — 0.458
  15. Orissa-India-print-ITW (PSP10074) — 0.455
  16. True-Albino-Teacher-ITW-WM (PSP10140) — 0.453
  17. Avery-Albino-ITW-WM (PSP10123) — 0.453
  18. Yeti (PSP10160) — 0.451
  19. DNA-PC-15SEPT2022 (RSP12860) — 0.450
  20. GoldenCoast-BAF-B1-UM (PSP10107) — 0.449

Sequence Data Downloads

The blockchain-registered VCF above can be downloaded by anyone, so its hash can be checked independently against the blockchain record. The remaining sequence data files — FASTQ, BAM and assemblies — are available to authorised accounts. Log in to download, or contact us for access.

Psilocybin

The psilocybin biosynthesis genes sit together in one cluster on chromosome 10 (psiD, psiK, psiM, psiH, psiR, psiT1, psiT2). Every assembled genome in Psilocydia was compared with the MGC_Penvy_1 reference across this window; below are the variants SkiBum carries there, and which other samples carry the same ones. This section compares assemblies only; the psilocybin gene cards under Variants combine the assembly with the reads, so they can list changes that are not here.

Loading the cluster variants…

The enzymes in 3D

This sample's version of each enzyme next to the P. Envy reference's. Flip between the two models; the track underneath shows every change seen in the collection and how many samples carry it, with this sample's changes filled in.

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